A reverse-phase, isocratic high-performance liquid chromatography assay for levofloxacin
نویسندگان
چکیده
منابع مشابه
High-performance liquid chromatography for hormone assay.
High-performance liquid chromatography (HPLC) is a refinement of traditional column chromatographic techniques. The speed of analysis and the resolution are increased with new column-packing materials and eluant pumped through the column at high pressure. The potential for achieving measurements of hormones in small volumes of plasma or urine is limited, both in normal and pathological situatio...
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Purpose: To develop a sensitive and rapid reverse phase high performance liquid chromatography (HPLC) method for the measurement of the levels of zidovudine (ZVD) and nevirapine (NVP) in human plasma. Methods: Standard stock solutions for HPLC analysis were prepared by dissolving ZVD and NVP in methanol. In the HPLC measurement, sample detection was carried out at 246 nm using an ultraviolet (U...
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Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...
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A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of Ramipril and amlodipine besylate in the combined pharmaceutical dosage form. The chromatographic separation for ramipril and amlodipine besylate were achieved with mobile phase containing mixed phosphate buffer (0....
متن کاملReverse-phase chromatography of polar biological substances: separation of catechol compounds by high-performance liquid chromatography.
Catecholamines and their metabolites have been separated isocratically by reverse-phase chromatography with aqueous (no organic solvent admixed) eluents. Unlike ion-exchange or ion-pair chromatography, mixtures of both acidic and basic substances can be separated in a single chromatographic run, because the retention is governed by hydrophobic interactions between the nonpolar moiety of the sol...
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ژورنال
عنوان ژورنال: Journal of Antimicrobial Chemotherapy
سال: 1999
ISSN: 1460-2091
DOI: 10.1093/jac/43.3.434